Volumen 105 - año 2019

Número 2 (abril)

Página 82

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Título / Title:

82 DETECTION OF SOME VIRULENCE FACTORS GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT CLINICAL CASES. D. Ghanim Al-Naaily, R. Masheel Salim, Aqeel Meer Al-Zamily.

Resumen / Abstract:

Staphylococcus aureus is one of the most important pathogenic type to humans, and the most common species responsible for a wide range of diseases such as furuncles, various abscesses, wounds abscesses resulting from surgical operations, dermatitis, soft tissue inflammation, arthritis, bones inflammation, bronchial pneumonia, inflammation of internal parts of the heart and injuries caused by toxins such as toxic shock syndrome and staphylococcus aureus syndrome and food poisoning. The current study aimed by finding the genes responsible for the virulence factors in S.aureus isolates by using the Single and Multiplex PCR mechanism (technology). A total of 60 specimens (urine, burn swabs, wound swabs) from different clinical cases were collected from patients (in different age groups) who admitted to several health centers in Al-Diwaniyah Teaching Hospital, Iraq, during a period extending from October 2016 to January 2017. Some virulence factors were investigated for 30 isolate only of MRSA using Single and Multiplex PCR for detection virulence factor genes which both coa gene encoding production of coagulase, clfA gene encoding for clumping factor, spa gene encoding for protein A, fnbA gene encoding for fibronectin binding proteins, luks gene encoding production of Panton Valentine Leukocidin(PVL). Results of current study showed that all isolates 30 (100%) were possess coa,clfA,spa and fnbA genes, 13 (43.3%) were possess luks gene.

Keyword: Virulence Factors; Staphylococcus Aureus; spa gene; clfA gene